Study on supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

Abstract Background Bovine tuberculosis (bTB), is a worldwide disease caused by Mycobacterium bovis ( M. ). The success of bTB eradication and control programs based on early detection the removal reactors from herd thus routine testing cull strategy have been applied globally. Since late nineteenth century, Tuberculin Skin Test (TST) has primary antemortem test available to support campaigns. Due TST limitations in terms Se Sp, credibility diagnosis frequently questioned given occurrence false-positive false-negative reactions, therefore, it necessary confirm reactive animals using other methods, ensuring reliability diagnosis. aim this study was evaluate sensitivity specificity multiplex enzyme-linked immunosorbent assay (ELISA) relative tuberculin used for cattle Brazil. Results Lack agreement between comparative cervical ELISA IDEXX TM observed. 2 positive did not react at 22 negative were TM. showed that significantly lower than official screening single tuberculin. also detected infected herds undetected test. parallel use increased feasibility screening. Conclusions results obtained here suggest may be supplemental infection regions without slaughter, where generally progresses more advanced stages antibody responses are likely prevalent. Evidence validation IDEXX™ as provided.